Accuris Hot Start Taq DNA Polymerase ensures controlled enzyme activation, preventing non-specific amplification and enabling room-temperature reaction setup. Bound with a monoclonal antibody, it remains inactive until heat-activated at 95°C. Ideal for low-copy PCR, multiplexing, and GC-rich templates, it is available in 5X buffer format or a ready-to-use 2X Master Mix, with an optional Red Dye Master Mix for direct gel loading.
Accuris Hot Start Taq DNA Polymerase is designed for high-specificity PCR applications, providing exceptional sensitivity and reliability. Unlike standard Taq polymerases, this enzyme is bound with a monoclonal antibody that blocks its activity until heat activation at 95°C. This prevents non-specific amplification and primer-dimer formation, allowing reaction setup at room temperature without compromising results.
Accuris Hot Start Taq is particularly well-suited for:
The polymerase is supplied with a 5X buffer, optimized for fast cycling and high reproducibility. For added convenience, it is also available in a 2X Master Mix, which simplifies PCR setup by combining the enzyme, buffer, and essential reaction components.
For users performing gel electrophoresis, the Red Dye Master Mix incorporates a built-in loading dye, allowing direct sample loading onto agarose gels. The red dye enhances pipetting visibility, and the buffer's density ensures proper well placement, reducing processing steps.
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